Efficacy of clinoptilolite supplementation on milk yield and somatic cell count

ABSTRACT Objective. To determine the efficiency of clinoptilolite supplements on milk production and somatic cell count (SCC). Materials and methods. 80 Holstein–Friesian cows were used, between 2 and 4 years of age ad between their first and third lactation. Two groups made up of 40 animals were constituted, and one of the following treatments were assigned randomly: Control group (n=40) with a basal diet, and experimental group (Clinoptilolite; n=40) with a basal diet + 3% (p/p) of clinoptilolite. The basal diet consisted of corn, hay, sunflower flour, barley grains, wheat bran and soy flour. The experiment lasted 16 weeks (February to June 2013) and began 4 weeks before the expected delivery date. 2560 milk samples were taken (morning and evening), and the farm was visited twice a week. Results. The mean values for the control group and the clinoptilolite group were 30.63±0.851 and 33.66±0.756, respectively. Milk prouction for the clinoptilolite group was higher than that of the control group (p<0.01). SCC for the control and clinoptilolite groups was 5.06±0.045 and 4.79±0.011, respectively (p<0.01). Conclusions. Supplementing with 3% (p/p) clinoptilolite in dairy cows increases milk production and decreases somatic cell count

Zeolite clinoptilolite nanoporous feed additive for animals of veterinary importance: potentials and limitations

In the past two decades many substances of natural or synthetic origin were studied as potential alternatives to antibiotic growth promoters (AGP) and some of them, particularly immunomodulators (IMs) and nutraceuticals (NCs), have shown to be capable of stimulating functions of the immune system and improving general health. At the same time, they were shown to be harmless for animals and the environment. Promising results have been obtained with natural clay minerals, zeolites among which clinoptilolite (CPL) is the best known as zootechnical and biomedical feed ingredient widely reported in scientific literature and used in farm animal nutrition. CPL has a potential to replace AGP due to its unique anti-bacterial properties, safety and efficacy as dietary supplement in food animals unifying potentials of an IM and NC. Currently, there are many reasons for CPL utilization in animal biotechnology and veterinary medicine because of it’s detoxificating, antioxidant, hemostatic, anti-diarrheic, growth-promoting and immunostimulating properties. Also, in human medicine it is an adjuvant in immunodeficiency states, oncology (after chemotherapy and radiotherapy) or reducer of radioactive elements. The aims of this review were to compile and discuss scientific data on safety and efficiency of nutritive modulation by dietary CPL (and other zeolites) as an alternative to AGP in animals of veterinary importance In particular the aim is to analyse its potentials and limitations in cattle regarding metabolic and endocrine status, oxidative stress and systemic/local inflammatory responses involved in reproductive and metabolic disorders of dairy cows. Altogether, these analyses will contribute to objective validation of practical significance of CPL as a novel feed additive able to maintain and improve health, fertility and performance in cattle production. </p

Dietary zeolite clinoptilolite supplementation influences chemical composition of milk and udder health in dairy cows

Cilj ovog istraživanja bio je ustanoviti utjecaj zeolita klinoptilolita (KPL) dodavanog u obroke mliječnih krava na kemijski sastav mlijeka, broj somatskih stanica (BSS) i zdravlje vimena. Dvadeset krava Holštajn-frizijske pasmine u dobi od 3 do 5 godina, gravidnih tri mjeseca i držanih u maloj obiteljskoj farmi u okolici Đurđevca, Hrvatska, bile su uključene u ovo istraživanje. Krave su bile nasumično razvrstane u dvije skupine, kontrolnu (n=10) i skupinu hranjenu s dodatkom KPL (n=10), i to svakodnevno sa 100 g zeolita u obroku za mliječne krave. Prvo uzorkovanje mlijeka načinjeno je prije dodavanja KPL u hranu. Još su četiri uzorkovanja provedena kroz četiri mjeseca, do sedmog mjeseca gravidnosti, odnosno suhostaja. Uzorci mlijeka bili su analizirani s obzirom na kemijski sastav mlijeka (mliječna mast, bjelančevine, laktoza, nemasna suha tvar i ureja), BSS i bakteriološkom pretragom. Uočene razlike u sadržaju pojedinog pretraživanog sastojka mlijeka nisu se značajno razlikovale, ni za pojedinačno uzorkovanje niti između pokusnih skupina krava. Međutim, kemijski sastav mlijeka bio je mnogo stabilniji u krava kojima je dodavan KPL u hranu. Naime, statistički značajna razlika između pojedinačnih uzorkovanja u krava kojima je dodavan KPL bila je utvrđena za sadržaj mliječne masti i ureje. Štoviše, u mlijeku krava iz kontrolne skupine utvrđene su značajne razlike između pojedinačnih uzorkovanja za sve pretražene sastojke mlijeka, s izuzetkom nemasne suhe tvari. Broj BSS u mlijeku krava iz obiju skupina nije se značajno razlikovao. Međutim, krave iz kontrolne skupine imale su 21 puta veći rizik od pojave intramamarnih infekcija nego krave kojima je KPL dodavan u hranu. Ovakav povoljni ishod naših istraživanja može se pripisati antibakterijskom, detoksikacijskom, antioksidativnom i imunostimulacijskom učinku KPL na metabolizam krava što se očitovalo padom pojavnosti intramamarnih infekcija tijekom suhostaja, porođaja i rane laktacije. Ovaj ishod vjerojatno bi se mogao objasniti ublažavanjem stresnih stanja tijekom spomenutih razdoblja kada su krave najosjetljivije na metaboličke poremećaje i nepovoljne okolišne uvijete koji rezultiraju u izraženijoj imunosupresiji i prijemljivosti za intramamarne infekcije.The aim of the current study was to establish the influence of dietary zeolite clinoptilolite (CPL) on the chemical composition of milk, somatic cell counts and udder health in dairy cows. Twenty cows of the Holstein-Frisian breed, aged between 3 and 5 years, which were pregnant for 3 months and kept in a small family farm in vicinity of Đurđevac, Croatia, were included in the trial. Cows were randomly assigned into two groups, control (n=10) and CPL-fed group (n=10). The CPL group received 100 g zeolite in the ratio for dairy cows on a daily basis. The first milk sampling was taken prior to adding CPL to feed. The four consecutive samplings were performed on a monthly basis up to 7th month of pregnancy, i.e., the dry period. The milk samples were analysed for chemical composition (milk fat, proteins, lactose, non- fatty dry matter and urea), somatic cell counts and by bacteriological examination. Observed differences in the content of particular milk components tested did not differ significantly between groups in any of sampling points. However, the chemical composition of milk was found to be more stable in CPL-fed cows. Statistically significant differences were found for milk fat and urea contents between single samplings in CPL-fed cows. Moreover, in the control group, significant differences were found between single samplings for all milk components, except non-fatty dry matter. The number of SCC in milk between the groups did not differ significantly. However, the control cows had a 21-fold higher odd of intramammary infections than CPL-fed cows. This beneficial outcome of the study may be attributed to the antibacterial, detoxifying, antioxidative and immunostimulating effects of CPL on the metabolism of cows, as exhibited by a decreased incidence of intramammary infections during the dry period, parturition and early lactation. Such an outcome might be explained by the moderation of stressful events accompanying such periods, when cows are the most sensitive to metabolic imbalance and environmental detrimental effects, resulting in more pronounced immunosuppression and susceptibility to intramammary infections

The effect of the dietary supplement zeolites clinoptilolite on somatic cell count, udder health and the chemical composition of milk in the cows of Holstein breed

U mliječnoj govedarskoj industriji posljednjih se godina nameće potreba za alternativnim dodacima u prehrani mliječnih krava radi kvalitetnije proizvodnje mlijeka i boljega zdravlja plotkinja. Cilj ovoga istraživanja bio je ustvrditi učinkovitost dodavanja vibroaktiviranog i mikroniziranog zeolita klinoptilolita (Vibrosorb®, Podpićan, Hrvatska) u hrani na kemijski sastav mlijeka i zdravlje vimena mliječnih krava. U istraživanje je uključeno 30 mliječnih krava holštajnske pasmine, dobi od 3 do 5 godina, gravidnih tri mjeseca u početku provedbe istraživanja. Životinje su bile držane na obiteljskom gospodarstvu u okolici Đurđevca u Hrvatskoj. Krave su nasumično podijeljene u dvije skupine: kontrolnu (KON, n = 15) i pokusnu (KPL, n = 15), koja je u obroku za mliječne krave svakodnevno dobivala i 100 g KPL-a. Prvo uzorkovanje mlijeka učinjeno je prije početka dodavanja KPL-a. Ostala četiri uzorkovanja provedena su tijekom četiri mjeseca do suhostaja, tj. sedmog mjeseca gravidnosti. Uzorci mlijeka analizirani su s obzirom na kemijski sastav mlijeka (mliječna mast, bjelančevine, laktoza, bezmasna suha tvar i ureja), broj somatskih stanica (BSS) i mikrobiološkom pretragom. Statističkom obradom ustvrđeno je da su prosječne vrijednosti kemijskog sastava mlijeka bile podjednake u obje skupine tijekom svih mjeseci, sa zanemarivom razlikom u 5. mjesecu u skupini KPL za mliječne bjelančevine. Statistički značajna razlika dobivena je za BSS (P 0,05). A statistically significant difference was found in the SCC; the CPL group had a significantly lower average value of SCC as opposed to the control group (P<0,05). The CPL-fed group had a 50% lower incidence of subclinical mastitis compared to the control group. Such a positive effect of CPL supplementation on udder health could indicate the immunostimulatory effect of vibroactivated and micronized clinoptilolite as a dietary supplement on the udder health of dairy cows

Production performance of dairy cows after the dietary addition of clinoptilolite

Clays are frequently proposed for the addition to dairy cattle diets to reduce the transfer of mycotoxins in milk. This study examined the effect of the addition of Clinoptilolite to the diet of lactating dairy cows on milk production and milk com- position. Thirty-two lactating Holstein cows (average lactation length: 142 d) were blocked according to milk production, parity, and days of lactation for assignment to one of two dietary treatments: control diet; control diet + Clinoptilolite, 200g/d. The control diet was based on corn and alfalfa silages, hay and concentrates, and did not contain aflatoxins above the safe level. The experimental period lasted 76 days. The Clinoptilolite supplementation did not affect milk yield and milk composition other than urea contents. The urea level in milk was negatively affected by Clinoptilolite addition (con- trol group 29.7 mg/100 ml vs Clinoptilolite group 31.3 mg/100ml). The dietary addition of Clinoptilolite did not change pH, ammonia content and VFA molar percentages in the rumen. No dietary effect on mineral contents of blood plasma (Na, K, Zn, and Ca) was observed. In case of clinoptilolite use in dairy cattle feeding, scarce negative effect on milk pro- duction and quality are expected

Mastitis u goveda: trajni i ponavljajući problem koji zahtijeva nove pristupe za njegovu kontrolu - pregledni članak

Mastitis in dairy cows is the inflammation of the mammary gland, and is the most important cause of economic losses in the modern dairy industry. Bovine mastitis is often infectious and can be caused by some 150 microbial species. The environment of the dairy cow is heavily colonized by microbiota, while milk is an ideal medium for propagation of the bacteria and causative agents. Staphylococcus aureus is the most common pathogen isolated from the mammary gland capable of causing health disorders in humans. Milk from an infected mammary gland has a different chemical composition compared to milk from healthy cows. Certain differences, such as protein components specific to inflamed tissue, are currently being considered in the development of diagnostic tools for mastitis. The known risk factors for mastitis can result in mastitis at the quarter, cow and herd levels. Mastitis treatment includes both preventive and therapy measures, and is primarily based on the use of antibiotics. Recent approaches to mastitis therapy have been focused on the design and use of natural therapeutics, such as zeolites, ozone and propolis, that could serve as alternatives to antibiotics.Mastitis mliječnih krava jest upala mliječne žlijezde i najvažniji je uzrok ekonomskih gubitaka u suvremenoj mliječnoj industriji. Najčešće je infektivne naravi, a može ga prouzročiti 150 različitih vrsta mikroba. Okoliš krave naseljen je obiljem mikrobiota, a mlijeko je idealna podloga za razmnožavanje bakterija i ostalih uzročnika. Najčešći uzročnik mastitisa izoliran iz mliječne žlijezde jest Staphylococcus aureus, koji može prouzročiti zdravstvene poremećaje i u ljudi. Mlijeko iz inficirane mliječne žlijezde kemijski je promijenjeno u odnosu na mlijeko iz zdrave četvrti. Neke od promjena, poput komponenti proteina karakterističnih za upale tkiva, znanost pokušava iskoristiti za dizajniranje novih dijagnostičkih testova za otkrivanje mastitisa. Dosad identificirani čimbenici rizika za mastitis mogu utjecati na njegovu pojavnost na razini četvrti, pojedine krave te stada. Liječenje mastitisa uključuje preventivne i terapijske mjere uglavnom pomoću antibiotika. U novije se vrijeme terapijski pristup mijenja uvođenjem prirodnih ljekovitih sredstava poput zeolita, ozona i propolisa, koji su potencijalne alternative antibioticima

SAFE USE OF BENTONITE IN DAIRY COWS. ASSESSMENT OF PHYSIOLOGICAL RESPONSES BY STUDYING METABOLOME AND MINERAL CONTENT IN BIOFLUIDS AND MILK CHARACTERISTICS.

La bentonite e altri minerali argillosi sono ampiamente utilizzati come additivi per mangimi negli allevamenti lattiferi per contrastare la contaminazione da micotossine dei mangimi, che influisce negativamente sulla salute e sulla produzione degli animali e rappresenta un grave rischio per i consumatori. Tuttavia, alcuni studi hanno riportato potenziali effetti indesiderati dei minerali argillosi in diverse specie animali e colture cellulari e, sulla base di osservazioni aneddotiche fatte dai casari, si teme che la bentonite possa influire negativamente sulle caratteristiche del latte vaccino e sulla produzione di formaggio. Inoltre, i minerali argillosi sembrano in grado di interagire con il microbiota intestinale e ruminale. Pertanto, lo scopo della presente tesi è stato quello di indagare i possibili effetti negativi della somministrazione di bentonite nelle vacche da latte. In particolare, sono stati condotti tre diversi esperimenti: due in vitro per valutare le potenziali interferenze di una bentonite commerciale sulle fermentazioni ruminali e uno in vivo su vacche da latte Holstein in lattazione per indagare i possibili effetti collaterali di questo additivo sulla salute, la fisiologia e la produzione degli animali e sulle caratteristiche e le proprietà casearie del latte. In questi studi sono stati applicati diversi metodi analitici, comprese le “Omics”. I risultati ottenuti suggeriscono che la bentonite può ridurre significativamente la produzione ruminale di gas in vitro, perlomeno alla dose più elevata utilizzata nell’esperimento. Inoltre, la bentonite ha prodotto lievi modifiche del metaboloma e del contenuto minerale (soprattutto microelementi) del liquido del rumine sia in vitro che in vivo, anche ai dosaggi stabiliti dalla normativa dell'Unione Europea. Tuttavia, i meccanismi omeostatici delle bovine sono sembrati in grado di contrastare questi lievi cambiamenti nel rumine, dal momento che sono stati riscontrati effetti trascurabili o nulli della bentonite sul metaboloma e sul contenuto minerale del plasma e del latte, nonché sugli altri parametri considerati (ossia indicatori generali della salute e della produzione animale, biomarcatori dello stress ossidativo, qualità e sottopopolazioni leucocitarie del latte, proteoma e peptidoma e proprietà di caseificazione del latte). In conclusione, la somministrazione di bentonite può essere considerata una pratica sicura negli allevamenti lattiferi in termini di salute e produzione animale, caratteristiche del latte per il consumo umano e proprietà di caseificazione del latte, almeno alle dosi prescritte dall'Unione Europea e per brevi periodi di trattamento. Sarebbero necessari ulteriori studi per valutare i possibili effetti collaterali della somministrazione a lungo termine della bentonite nelle vacche da latte.Bentonite and other clay minerals are largely used as feed additives in dairy farms in order to counteract the mycotoxin contamination of feed, which negatively affects animal health and production and represents a serious risk for consumers. However, some studies reported potential undesirable effects of clay minerals in different animal species and cell cultures and, based on anecdotical observations made by cheesemakers, there is concern that bentonite may negatively affect cow milk characteristics and cheesemaking. Moreover, clay minerals appear capable of interacting with the gut and ruminal microbiota. Therefore, the aim of the present dissertation was to investigate possible negative effects of bentonite administration in dairy cows. In particular, three different experiments were conducted: two in vitro experiments evaluated potential interferences of a commercial bentonite on ruminal fermentations and one in vivo experiment was performed in lactating Holstein dairy cows to investigate any possible side effects of this additive on animal health, physiology, and production and on milk characteristics and cheesemaking properties. In these studies, many different analytical methods were applied, including Omics. The results obtained suggest that bentonite can significantly reduce ruminal gas production in vitro, at least at the higher dose used in the experiment. Moreover, bentonite produced slight modifications of the metabolome and mineral content (mainly microelements) of the rumen fluid both in vitro and in vivo, also at the dosages set by the European Union regulation. However, cows’ homeostatic mechanisms seemed able to counteract these slight changes in rumen, as negligible or no effects of bentonite were found on the metabolome and mineral content of plasma and milk as well as on the others parameters considered (i.e. general indicators of animal health and production, oxidative stress biomarkers, milk quality and leukocyte subpopulations, milk proteome and peptidome, and milk cheesemaking properties). In conclusion, bentonite administration can be considered a safe practice in dairy farms in terms of animal health and production, milk characteristics for human consumption, and milk cheesemaking properties, at least at the doses prescribed by the European Union and for short periods. Further studies would be needed to evaluate possible side effects of long-term administration of bentonite in dairy cows

Učinak dodatka prehrani na subklinički mastitis krava

Bovine mastitis is an important commercial and sanitary cost in milk production. The prevention and treatment of this disease is essentially based on the use of antibiotics that have limited effectiveness and can negatively affect milk quality. The aim of this study was to evaluate the effectiveness of an alternative treatment based on a symbiotic in the control and prevention of subclinical mastitis (SCM), and in the improvement of the quality of milk produced on a dairy farm in north-central Algeria. The milk of 68 cows was collected and analysed by somatic cell count and bacteriological analysis. Three samples were taken at one-month intervals. The first sample was taken before the administration of a feed additive (SYMBIOVEBA®) to lactating dairy cows. Animals were divided into two groups to study the effects (curative and preventive) of the symbiotic. Each group was further divided into two subgroups, where one received the symbiotic and the other was the control. The prevalence of subclinical mastitis at this farm was 33.82%. Bacterial identification was performed using classical methods only on the group of cows with mastitis; a total of 13 bacterial species were isolated from the three samples. Staphylococci were dominant, with a frequency of 45%, followed by Enterobacteriaceae (40%). These rates were considerably lower in both subgroups, though treated cows showed 100% cure rate for both Staphylococci and Enterobacteriaceae. Individual somatic cell count (ISCC) was performed on all three samples and in both groups, and revealed a cure threshold (S) of 200,000 cells/mL, and a 100% cure rate for the SYMBIOVEBA®-treated cow subgroup compared to 62.5% for the control subgroup (P<0.05).Mastitis krava predstavlja važan ekonomski i sanitarni trošak u proizvodnji mlijeka. Prevencija i liječenje ove bolesti u osnovi se temelje na uporabi antibiotika koji negativno utječu na kakvoću mlijeka i čija je učinkovitost ograničena. Cilj je ove studije bio procijeniti učinkovitost alternativnog liječenja na temelju simbioze kontrole i prevencije subkliničkog mastitisa (SCM) te poboljšanja kakvoće mlijeka koje se proizvodi na farmi mlijeka koja se nalazi u sjevernom središtu Alžira. Prikupljeno je mlijeko 68 krava te analizirano prema broju somatskih stanica, a učinjena je i bakteriološka analiza. Izuzeta su tri različita uzorka u intervalu od mjesec dana. Prvi je uzorak izuzet prije davanja dodatka prehrani (SYMBIOVEBA®) mliječnim kravama u laktaciji. Životinje su klasificirane u 2 skupine kako bi se proučila dva učinka (ljekoviti i preventivni) simbioze. Svaka skupina podijeljena je u dvije skupine, jedna je primala simbiozu, a druga je bila kontrolna skupina. Pojavnost subkliničkog mastitisa na ovoj farmi bila je 33,82 %. Identifikacija mikroorganizama provedena je klasičnom metodom i odnosila se samo na skupinu krava s mastitisom na početku; to je dopustilo izolaciju 13 različitih mikroorganizama iz tri uzastopno uzorkovana uzorka. Otkrivena je dominacija stafilokoka s učestalošću od 45 % i enterobakterija (40 %). Ovi su postotci bili značajno niži u obje skupine, osim što je to bilo značajnije u liječenih krava: 100 % izlječenja za stafilokoke i enterobakterije. Broj somatskih stanica u mlijeku pojedine krave (ISCC) ispitan je u sva tri uzorka i u obje skupine je otkrio, za prag uprabe terapije (S) od 200,000 stanica/mL, 100 % postotak izlječenja za skupinu krava koje su primale SYMBIOVEBA® dodatak prehrani u usporedbi sa 62,5 % za kontrolnu skupinu krava (P<0,05)

Antioksidacijski učinak nove intramamarne formulacije na bazi pčelinjih proizvoda Apimasta® u muznih krava.

The aim of this study was to determine the antioxidant effect of the novel bee-product based intramammary formulation Apimast® on the oxidative status of dairy cows. Apimast® was administered intramammarily three times at 12 h intervals to 10 dairy cows on each of the three dairy farms. The oxidative status markers and some metabolic parameters were determined in the milk (GSH-Px, d-ROM, SOD, BAP, TAS) and in the serum (SOD, GSH-Px, NEFA, BHB, glucose). The results showed that the effect of Apimast® was mostly local, regardless of the concentration used (3% and 1%) as there was an increase in the concentration of GSH-Px and TAS in the milk, but not in the blood. It is also of a short-term nature since the values of these parameters were significantly lower 7 days after application. The expected connection between the increase of ROM in milk samples from the microbiologically positive quarters was not found. The results of this study confirm the local antioxidant effect of Apimast® in the udder, with implications for non-antibiotic subclinical mastitis treatment.Cilj istraživanja bio je odrediti učinak nove intramamarne formulacije na bazi pčelinjih proizvoda, Apimasta®, na oksidacijski status muznih krava. Apimast® je apliciran u tri navrata s razmacima od 12 sati u 10 krava na tri različite farme. Izmjereni su markeri oksidacijskog statusa kao i određeni metabolički pokazatelji u mlijeku (GSH-Px, d-ROM, SOD, BAP, TAS) i serumu (SOD, GSH-Px, NEFA, BHB, glukoza). Učinak Apimasta® bio je uglavnom lokalan, bez obzira na primijenjenu koncentraciju (1 i 3 %), jer porast koncentracija GSH-Px i TAS-a u mlijeku nije pratio porast u serumu. Promjene svih mjerenih pokazatelja bile su kratkotrajne i nakon sedam dana vraćale su se na približne vrijednosti početnih koncentracija. U bakteriološki pozitivnim četvrtima vimena nije zabilježen porast ROM-a. Ovo istraživanje potvrđuje lokalni antioksidacijski učinak Apimasta® u vimenu, što je relevantno s aspekta neantibiotskog liječenja supkliničkog mastitisa

Efectos de la suplementación clinoptilolita corto plazo sobre la ganancia de peso en becerros Holstein

Objective. To determine whether short term supplemantation at two levels (1 or 2 g/kg) of clinoptilolite (CLNP) in the colostrum of dairy calves had any effect on total weight gain (TWG) and mean daily gain of weight (mdwg). Materials and methods. A total of 24 clinically healthy calves without diarrhea were assigned in a subset of three major groups of calves were studied: group I (n=8) received colostrum with clnp at the rate of 1/kg started immediately following calving at 12 and 24 hours, for a total of 2 doses. Group II consisted of calves (n=8) receiving colostrum with clnp at the rate of 2/kg within the same interval as the first experimental group. The last group III, which served as control, received solely colostrum. Results. Group had significant effect on weight (kg) of Holstein calves [twg (mean±SE)- control: 12.66±0.349, group I: 14.73±0.414, group II: 14.19±0.468, p&lt;0.01; mdwg (mean±SE)-control: 0.338±0.0155, group I: 0.396±0.0189, group II: 0.397±0.0196, p&lt;0.05]. There was a significant difference observed for twg (p&lt;0.01) and mdwg (p&lt;0.05) among control group and both clnp treatment groups. Conclusions. The results showed that the addition of both levels of clinoptilolite administration via colostrum appeared to enhance twg and mdwg in newborn calves without having any observable adverse effect.Objetivo. Determinar si la suplementación a corto plazo en 2 niveles (1 o 2 g/kg) de clinoptilolita (CLNP) en el calostro de terneros lecheros tiene algún efecto sobre la ganancia de peso total (TWG) y la ganancia media diaria de peso (MDWG). Materiales y métodos. Se asignó un total de 24 terneros clínicamente sanos sin diarrea en un subconjunto de los tres grupos principales de terneros que fueron estudiados: grupo I (n = 8) recibieron calostro con CLNP a razón de 1 g/kg inmediatamente después del parto a las 12 y 24 horas , para un total de 2 dosis. Grupo II consistió de terneros (n = 8) que recibieron calostro con CLNP a razón de 2 kg / dentro del mismo intervalo que el primer grupo experimental. El último grupo III, que sirvió como control, recibió únicamente calostro. Resultados. Grupo tuvo un efecto significativo sobre el peso (kg) de terneros Holstein [GTT (media±DE) - Control: 12.66 ± 0.349, el grupo I: 14.73 ± 0.414, el grupo II: 14.19±0.468, p&lt;0.01; mdwg (media±DE) - Control: 0.338±0.0155, el grupo I: 0.396±0.0189, grupo II: 0.397 ± 0.0196, p&lt;0.05]. Hubo una diferencia significativa para TWG (p&lt;0.01) y mdwg (p&lt;0.05) entre el grupo control y ambos grupos de tratamiento CLNP. Conclusiones. Los resultados mostraron que la adición de los dos niveles de clinoptilolita a través del calostro mejoró TWG y MDWG en terneros recién nacidos sin tener ningún efecto adverso observable